Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR.

@article{Cassaing2006ComparisonBT,
  title={Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR.},
  author={Sophie Cassaing and M. H. B{\'e}ssi{\`e}res and Antoine Berry and Alexander A Berrebi and Richard Fabre and Jean-François Magnaval},
  journal={Journal of clinical microbiology},
  year={2006},
  volume={44 3},
  pages={720-4}
}
PCR is now commonly applied to the diagnosis of toxoplasmosis. Although several methods are available, comparative studies are few, making it difficult to compare the performance of each technique. We compared the sensitivities of two real-time PCR assays through a prospective study on fetuses, neonates, and immunocompromised patients and on the ocular diagnosis of toxoplasmosis. The first system targeted the widely used B1 gene (GenBank accession number AF179871) while the second (RE) targeted… CONTINUE READING

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