Comparison between terminal-restriction fragment length polymorphism (T-RFLP) and quantitative culture for analysis of infants' gut microbiota.

@article{Sjberg2013ComparisonBT,
  title={Comparison between terminal-restriction fragment length polymorphism (T-RFLP) and quantitative culture for analysis of infants' gut microbiota.},
  author={Fei Sj{\"o}berg and Forough L. Nowrouzian and Ignacio Rangel and Charles Hannoun and Edward Moore and Ingegerd Adlerberth and Agnes E. Wold},
  journal={Journal of microbiological methods},
  year={2013},
  volume={94 1},
  pages={
          37-46
        }
}
The infantile intestinal microbiota is a major stimulus for immune maturation. Both culture and DNA-based methods can be used for microbiota characterization, but few studies have systematically compared their performance for analysis of the gut microbiota. Here, we examined fecal samples obtained on six occasions between one week and 12 months of age from six vaginally delivered infants. After quantitative aerobic and anaerobic culture of the samples on selective and non-selective media, DNA… CONTINUE READING
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