Comparison between different strategies of covalent attachment of DNA to glass surfaces to build DNA microarrays.

@article{Zammatteo2000ComparisonBD,
  title={Comparison between different strategies of covalent attachment of DNA to glass surfaces to build DNA microarrays.},
  author={Nathalie Zammatteo and Louis Jeanmart and Sandrine Hamels and Sophie Courtois and Pierre Louette and L{\'a}szl{\'o} Hevesi and Jos{\'e} Remacle},
  journal={Analytical biochemistry},
  year={2000},
  volume={280 1},
  pages={143-50}
}
DNA microarray is a powerful tool allowing simultaneous detection of many different target molecules present in a sample. The efficiency of the array depends mainly on the sequence of the capture probes and the way they are attached to the support. The coupling procedure must be quick, covalent, and reproducible in order to be compatible with automatic spotting devices dispensing tiny drops of liquids on the surface. We compared several coupling strategies currently used to covalently graft DNA… CONTINUE READING