Comparison between Real-Time Quantitative PCR Detection of HER2 mRNA Copy Number in Peripheral Blood and ELISA of Serum HER2 Protein for Determining HER2 Status in Breast Cancer Patients

@inproceedings{Savino2009ComparisonBR,
  title={Comparison between Real-Time Quantitative PCR Detection of HER2 mRNA Copy Number in Peripheral Blood and ELISA of Serum HER2 Protein for Determining HER2 Status in Breast Cancer Patients},
  author={Maria Savino and Paola Parrella and Massimiliano Copetti and Raffaela Barbano and Roberto Murgo and Vito Michele Fazio and V. Valori and Massimo Carella and Maria Garrubba and Stefano Angelo Santini},
  booktitle={Cellular oncology : the official journal of the International Society for Cellular Oncology},
  year={2009}
}
BACKGROUND The development of non-invasive procedure to determine HER2 status may represent a powerful method for monitoring disease progression and response to the treatment. METHODS Serum samples and RNA from peripheral blood were evaluated in 85 breast cancer patients (49 HER2 positive and 36 HER2 negative) and 22 healthy controls. HER2 mRNA levels were measured by real-time quantitative PCR (QPCR) and serum HER2 protein by immunoenzimatic assay (EIA). ROC curve analyses were used to… CONTINUE READING