Comparison and evaluation of two diagnostic methods for detection of npt II and GUS genes in Nicotiana tabacum.

Abstract

To diminish the time required for some diagnostic assays including polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP) and also a visual detection protocol on the basis of npt II and GUS genes in transgenic tobacco plants were used. Agrobacterium tumefaciens-mediated transformation of Nicotiana tabacum leaf discs was performed… (More)
DOI: 10.1007/s12010-015-1529-y

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