Comparison and applications of label-free absolute proteome quantification methods on Escherichia coli.

@article{Arike2012ComparisonAA,
  title={Comparison and applications of label-free absolute proteome quantification methods on Escherichia coli.},
  author={Liisa Arike and Kaspar Valgepea and Lauri Peil and Ranno Nahku and Kaarel Adamberg and Raivo Vilu},
  journal={Journal of proteomics},
  year={2012},
  volume={75 17},
  pages={5437-48}
}
Three different label-free proteome quantification methods--APEX, emPAI and iBAQ--were evaluated to measure proteome-wide protein concentrations in the cell. All the methods were applied to a sample from Escherichia coli chemostat culture. A Pearson squared correlation of approximately 0.6 among the three quantification methods was demonstrated. Importantly, the sum of quantified proteins by iBAQ and emPAI corresponded with the Lowry total protein quantification, demonstrating applicability of… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 46 extracted citations

Similar Papers

Loading similar papers…