Comparison and accuracy of methods to determine the confocal volume for quantitative fluorescence correlation spectroscopy.

@article{Rttinger2008ComparisonAA,
  title={Comparison and accuracy of methods to determine the confocal volume for quantitative fluorescence correlation spectroscopy.},
  author={Steffen R{\"u}ttinger and Volker Buschmann and Benedikt Kraemer and R. Erdmann and R. Macdonald and Felix Koberling},
  journal={Journal of microscopy},
  year={2008},
  volume={232 2},
  pages={343-52}
}
Single molecule detection based on fluorescent labels offers the possibility to gain not only qualitative but also quantitative insight into specific functions of complex biological systems. Fluorescence correlation spectroscopy is one of the favourite techniques to determine concentrations and diffusion constants as well as molecular brightness of molecules in the pico- to nano-molar concentration range, with broad applications in biology and chemistry. Although fluorescence correlation… CONTINUE READING