Comparison and Optimization of hiPSC Forebrain Cortical Differentiation Protocols

@inproceedings{Muratore2014ComparisonAO,
  title={Comparison and Optimization of hiPSC Forebrain Cortical Differentiation Protocols},
  author={Christina R. Muratore and Priya Srikanth and Dana G. Callahan and Tracy L Young-Pearse},
  booktitle={PloS one},
  year={2014}
}
Several protocols have been developed for human induced pluripotent stem cell neuronal differentiation. We compare several methods for forebrain cortical neuronal differentiation by assessing cell morphology, immunostaining and gene expression. We evaluate embryoid aggregate vs. monolayer with dual SMAD inhibition differentiation protocols, manual vs. AggreWell aggregate formation, plating substrates, neural progenitor cell (NPC) isolation methods, NPC maintenance and expansion, and astrocyte… CONTINUE READING

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