Comparing the antibody responses against recombinant hemagglutinin proteins of avian influenza A (H5N1) virus expressed in insect cells and bacteria.

@article{Shen2008ComparingTA,
  title={Comparing the antibody responses against recombinant hemagglutinin proteins of avian influenza A (H5N1) virus expressed in insect cells and bacteria.},
  author={Shuo Shen and Geetha Mahadevappa and Hsueh-Ling Janice Oh and Boon Yu Wee and Yook-Wah Choi and Le-Ann Hwang and Seng Gee Lim and Wanjin Hong and Sunil K. Lal and Y R Tan},
  journal={Journal of medical virology},
  year={2008},
  volume={80 11},
  pages={1972-83}
}
The hemagglutinin (HA) of influenza A virus plays an essential role in mediating the entry of the virus into host cells. Here, recombinant full-length HA5 protein from a H5N1 isolate (A/chicken/hatay/2004(H5N1)) was expressed and purified from the baculovirus-insect cell system. As expected, full-length HA5 elicits strong neutralizing antibodies, as evaluated in micro-neutralization tests using HA5 pseudotyped lentiviral particles. In addition, two fragments of HA5 were expressed in bacteria… CONTINUE READING

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