Comparing reagents for efficient transfection of human primary myoblasts: FuGENE 6, Effectene and ExGen 500

  title={Comparing reagents for efficient transfection of human primary myoblasts: FuGENE 6, Effectene and ExGen 500},
  author={A. S. Arnold and Vincent Laporte and Serge Dumont and Aline Appert-Collin and Patrick Erbacher and Gilliane Coupin and Rachel Levy and Philippe Poindron and J. -P. Gies},
  journal={Fundamental \& Clinical Pharmacology},
This study compared three different synthetic reagents (FuGENE 6, Effectene and ExGen 500) for the transfection of human primary myoblasts. We examined the efficiency, cytotoxicity and size of the complexes formed in the presence of different amounts of vector and DNA and with variable amounts of serum. Transfection rates were relatively high for primary cells, especially with FuGENE 6 (20%), which appeared to be the best transfection reagent for these cells, even in the presence of 10% serum… 

Hyperbranched polylysine: a versatile, biodegradable transfection agent for the production of recombinant proteins by transient gene expression and the transfection of primary cells.

HBPL-mediated transfection does not require complex pre-formation, works well in serum-containing media and is biodegradable, which may prevent cumulative cytotoxicity and facilitates downstream processing.

Transfection Efficiency and Cytotoxicity of Transfection Reagents in Human Umbilical Vein Endothelial Cells

It was established that FuGENE ® HD in the ratio of 5 : 2 was the most effective of the four reagents used for HUVEC line transfection, and the CMV promoter had a higher ability to express the reporter gene (EGFP) than the SV40 promoter.

Lipid‐based transfection as a method for gene delivery in zebrafish (Danio rerio) embryos

This work presents an approach to implement lipid-based transfection with two different reporter vectors as a faster, cheaper and more practical alternative than microinjection in zebrafish embryos at different developmental stages.

[Efficient production of transfected human keratinocytes under serum-free and feeder layer-free conditions].

  • C. RadtkeK. ReimersC. AllmelingP. Vogt
  • Biology, Medicine
    Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Mikrochirurgie der Peripheren Nerven und Gefasse : Organ der V...
  • 2009
An efficient and inexpensive method for a standardized human keratinocyte isolation without the need of a fibroblast feeder layer is described, which may facilitate the clinical application of cell based therapies in burn injuries or chronic wounds using keratinocytes.

Transfection types, methods and strategies: a technical review

With the vast choices of approaches available, it is hoped that this review will help researchers, especially those new to the field, in their decision making over the transfection protocol or strategy appropriate for their experimental aims.

Comparison and Optimisation of Transfection of Human Dental Follicle Cells, a Novel Source of Stem Cells, with Different Chemical Methods and Electro-poration

Electro-poration of HDFCs yield relatively high transfection rates and cell viability when compared to chemical transfections techniques, which might be useful for developing gene and cell therapy applications using dental follicle stem cells.

In-vivo gene transfer induces transgene expression in cells and secretions of the mouse cauda epididymis.

Mouse cauda epididymis were in-vivo transfected using the lipid FuGENE 6 as gene vector and the use of the pSEAP-control gene showed that cauda anesthesia secretions can also be modified by the transfection procedure.

Characterization of counterion effects of gemini surfactants and in vitro studies of transfection efficiency for gene therapy in epithelial ovarian cancer.

Gene therapy has emerged as a promising strategy for the treatment or prevention of many acquired or genetic diseases that are considered incurable at the present time. Although viral and non-viral

Foreign Gene Expression in the Mouse Cauda Epididymis is Regulated by Androgens

  • Pedro Esponda
  • Biology
    International Journal of Medical and Surgical Sciences
  • 2018
The possibility to improve transfection efficiency would increase the knowledge on epididymal physiology and would permit to modify the fertilizing capacity in mammals.



Transfection of cultured myoblasts in high serum concentration with DODAC:DOPE liposomes

A novel liposome formulation, DODAC:DOPE (1:1) is totally resistant to the inhibitory effects of serum for transfection of cultured myoblasts and myotubes and may be valuable for the systemic delivery of genetic information to muscle and other tissues.

Transfection of large plasmids in primary human myoblasts

An efficient and reproducible method to permit efficient delivery of large plasmids to human primary myoblasts for the ex vivo gene therapy of Duchenne muscular dystrophy is provided.

High efficiency transfection of primary skeletal muscle cells with lipid‐based reagents

Lipofection is a convenient method for gene transfer into muscle cells but reportedly is inefficient, but lipid‐based transfection into primary skeletal muscle cells can be several times more efficient than previously reported.

Lipofection of cultured mouse muscle cells: a direct comparison of Lipofectamine and DOSPER

A direct in vitro comparison of two commercially available cationic lipid formulations, Lipofectamine and DOSPER, found both were able to promote efficient, reproducible gene transfer in C2C12 cells, and to transfect primary mouse myoblast cultures successfully.

Analysis of differential lipofection efficiency in primary and established myoblasts.

The findings suggest that the lower transfectability of primary myoblasts is mostly due to a difference in the intracellular delivery pathway that correlates with more rapid delivery of internalized complex to the lysosomal compartment.

Liposome‐Mediated Gene Transfer into Normal and Dystrophin‐Deficient Mouse Myoblasts

In vitro studies indicate that cationic liposomes can be used to deliver recombinant genes to muscle cells at high efficiency and form a basis to expand investigations into in vivo expression of recombinant dystrophin protein either by direct intramuscular gene transfer or via implantation of transfected myoblasts.

Immunobiology and the future of myoblast transfer therapy.

This review on the immunological aspects of MTT focuses in particular on the roles of specific components of the host immune response, the effects of tissue culture on donor cells, and strategies under development to circumvent the problem of donor myoblast death after injection in vivo.

Transplantation of normal and DMD myoblasts expressing the telomerase gene in SCID mice.

Results demonstrate that the forced expression of telomerase does not block the ability of normal or dystrophic myoblasts to differentiate in vivo, and it will be now necessary to determine the factors that prevent telomersase from extending the life span of human myoblast before the potential of this intervention can be fully examined.

Gene transfer in skeletal muscle by systemic injection of DODAC lipopolyplexes

Analysis of reporter gene expression (luciferase) showed that regenerating muscle is more efficiently transfected in all cases and that IA injection is by far the best approach.

Development of Approaches to Improve Cell Survival in Myoblast Transfer Therapy

The results suggest that selection of specific muscle-derived cell populations or the control of inflammation can be used as an approach to improve cell survival after both myoblast transplantation and the myOBlast-mediated ex vivo gene transfer approach.