Comparing reagents for efficient transfection of human primary myoblasts: FuGENE 6, Effectene and ExGen 500

@article{Arnold2006ComparingRF,
  title={Comparing reagents for efficient transfection of human primary myoblasts: FuGENE 6, Effectene and ExGen 500},
  author={A. S. Arnold and Vincent Laporte and Serge Dumont and Aline Appert-Collin and Patrick Erbacher and Gilliane Coupin and Rachel Levy and Philippe Poindron and J. -P. Gies},
  journal={Fundamental \& Clinical Pharmacology},
  year={2006},
  volume={20}
}
This study compared three different synthetic reagents (FuGENE 6, Effectene and ExGen 500) for the transfection of human primary myoblasts. We examined the efficiency, cytotoxicity and size of the complexes formed in the presence of different amounts of vector and DNA and with variable amounts of serum. Transfection rates were relatively high for primary cells, especially with FuGENE 6 (20%), which appeared to be the best transfection reagent for these cells, even in the presence of 10% serum… 

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