Comparing de novo and reference-based transcriptome assembly strategies by applying them to the blood-sucking bug Rhodnius prolixus.

@article{Marchant2016ComparingDN,
  title={Comparing de novo and reference-based transcriptome assembly strategies by applying them to the blood-sucking bug Rhodnius prolixus.},
  author={Axelle Marchant and Florence Mougel and Vander Mendonça and Marion Quartier and Emmanuelle Jacquin-Joly and Jo{\~a}o Aristeu da Rosa and Emmanuelle Petit and Myriam Harry},
  journal={Insect biochemistry and molecular biology},
  year={2016},
  volume={69},
  pages={
          25-33
        }
}
High Throughput Sequencing capabilities have made the process of assembling a transcriptome easier, whether or not there is a reference genome. But the quality of a transcriptome assembly must be good enough to capture the most comprehensive catalog of transcripts and their variations, and to carry out further experiments on transcriptomics. There is currently no consensus on which of the many sequencing technologies and assembly tools are the most effective. Many non-model organisms lack a… CONTINUE READING
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