Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus

@article{Bartels2014ComparingWS,
  title={Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus},
  author={M. D. Bartels and A. Petersen and P. Worning and J. Nielsen and H. Larner-Svensson and H. Johansen and L. Andersen and J. Jarl{\o}v and K. Boye and A. Larsen and H. Westh},
  journal={Journal of Clinical Microbiology},
  year={2014},
  volume={52},
  pages={4305 - 4308}
}
ABSTRACT spa typing of methicillin-resistant Staphylococcus aureus (MRSA) has traditionally been done by PCR amplification and Sanger sequencing of the spa repeat region. At Hvidovre Hospital, Denmark, whole-genome sequencing (WGS) of all MRSA isolates has been performed routinely since January 2013, and an in-house analysis pipeline determines the spa types. Due to national surveillance, all MRSA isolates are sent to Statens Serum Institut, where the spa type is determined by PCR and Sanger… Expand
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References

SHOWING 1-10 OF 11 REFERENCES
Typing of Methicillin-Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database Management
ABSTRACT The spa gene of Staphylococcus aureus encodes protein A and is used for typing of methicillin-resistant Staphylococcus aureus (MRSA). We used sequence typing of the spa gene repeat region toExpand
Bacterial Whole-Genome Sequencing Revisited: Portable, Scalable, and Standardized Analysis for Typing and Detection of Virulence and Antibiotic Resistance Genes
ABSTRACT Multidrug-resistant nosocomial pathogens present a major burden for hospitals. Rapid cluster identification and pathogen profiling, i.e., of antibiotic resistance and virulence genes, areExpand
A pilot study of rapid benchtop sequencing of Staphylococcus aureus and Clostridium difficile for outbreak detection and surveillance
TLDR
This benchtop sequencing technology is widely generalisable to human bacterial pathogens and provides several good examples of how rapid and precise sequencing could transform identification of transmission of healthcare-associated infection and therefore improve hospital infection control and patient outcomes in routine clinical practice. Expand
Rapid detection, differentiation and typing of methicillin-resistant Staphylococcus aureus harbouring either mecA or the new mecA homologue mecA(LGA251).
  • M. Stegger, P. Andersen, +7 authors A. Larsen
  • Biology, Medicine
  • Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
  • 2012
TLDR
Over a 2-month period, 12 new MRSA cases caused by isolates carrying mecA(LGA251) were identified, emphasizing the clinical importance of testing for these new MR SA isolates. Expand
Based Upon Repeat Pattern (BURP): an algorithm to characterize the long-term evolution of Staphylococcus aureus populations based on spa polymorphisms
TLDR
Based Upon Repeat Pattern is the first automated and objective tool to infer clonal relatedness from spa repeat regions and is able to extract an evolutionary signal rather congruent to MLST and micro-array data. Expand
Community-associated MRSA: what makes them special?
  • M. Otto
  • Biology, Medicine
  • International journal of medical microbiology : IJMM
  • 2013
TLDR
Interestingly, CA-MRSA strains emerged globally and from different backgrounds, indicating that the "trade-off" between maintaining sufficient levels of methicillin resistance and obtaining enhanced virulence at a low fitness cost was achieved on several occasions in convergent evolution. Expand
High Interlaboratory Reproducibility of DNA Sequence-Based Typing of Bacteria in a Multicenter Study
TLDR
DNA sequence-based typing of the Staphylococcus aureus protein A gene showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureUS DNA samples sent to 10 laboratories. Expand
Emergence and resurgence of meticillin-resistant Staphylococcus aureus as a public-health threat
TLDR
Of all the resistance traits S aureus has acquired since the introduction of antimicrobial chemotherapy in the 1930s, meticillin resistance is clinically the most important, since a single genetic element confers resistance to the most commonly prescribed class of antimicrobials--the beta-lactam antibiotics, which include penicillins, cephalosporins, and carbapenems. Expand
Transforming clinical microbiology with bacterial genome sequencing
TLDR
It is predicted that the application of next-generation sequencing will soon be sufficiently fast, accurate and cheap to be used in routine clinical microbiology practice, where it could replace many complex current techniques with a single, more efficient workflow. Expand
Beware of mis-assembled genomes
TLDR
It is observed that even the best assemblers today make exactly this mistake when assembling the Drosophila species currently being sequenced, and developed software for improving assemblies that can detect at least some situations like the one shown above. Expand
...
1
2
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