Comparing COI and ITS as DNA Barcode Markers for Mushrooms and Allies (Agaricomycotina)

  title={Comparing COI and ITS as DNA Barcode Markers for Mushrooms and Allies (Agaricomycotina)},
  author={Bryn T. M. Dentinger and Maryna Ya. Didukh and Jean-Marc Moncalvo},
  booktitle={PloS one},
DNA barcoding is an approach to rapidly identify species using short, standard genetic markers. The mitochondrial cytochrome oxidase I gene (COI) has been proposed as the universal barcode locus, but its utility for barcoding in mushrooms (ca. 20,000 species) has not been established. We succeeded in generating 167 partial COI sequences (~450 bp) representing ~100 morphospecies from ~650 collections of Agaricomycotina using several sets of new primers. Large introns (~1500 bp) at variable… CONTINUE READING
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