Comparative study of the cytolytic activity of myotoxic phospholipases A2 on mouse endothelial (tEnd) and skeletal muscle (C2C12) cells in vitro.

@article{Lomonte1999ComparativeSO,
  title={Comparative study of the cytolytic activity of myotoxic phospholipases A2 on mouse endothelial (tEnd) and skeletal muscle (C2C12) cells in vitro.},
  author={Bruno Lomonte and Yamileth Angulo and S. Rufini and Wonhwa Cho and Jos{\'e} Roberto Giglio and Motonori Ohno and Jos{\'e} J Daniele and P M Geoghegan and Jos{\'e} Mar{\'i}a Guti{\'e}rrez},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={1999},
  volume={37 1},
  pages={145-58}
}
A rapid in vitro cytolytic effect of some myotoxic phospholipases A2 (PLA2s) isolated from the venoms of Viperidae snakes has been previously described. This study was undertaken to investigate if cytolytic activity is a common property of the myotoxic proteins from this group. Murine endothelial cells (tEnd) and skeletal muscle myotubes (C2C12) were utilized as targets. The release of lactic dehydrogenase was quantified as a measure of cell damage, 3 h after exposure of cells to the different… CONTINUE READING