Comparative study of sensitivity, linearity, and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus.

@article{Nixon2014ComparativeSO,
  title={Comparative study of sensitivity, linearity, and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus.},
  author={Gavin J. Nixon and Jeremy A. Garson and Paul Grant and Eleni Nastouli and Carole A. Foy and Jim F Huggett},
  journal={Analytical chemistry},
  year={2014},
  volume={86 9},
  pages={4387-94}
}
Performing nucleic acid amplification techniques (NAATs) in digital format using limiting dilution provides potential advantages that have recently been demonstrated with digital polymerase chain reaction (dPCR). Key benefits that have been claimed are the ability to quantify nucleic acids without the need of an external calibrator and a greater resistance to inhibitors than real-time quantitative PCR (qPCR). In this study, we evaluated the performance of four NAATs, qPCR, dPCR, real-time… CONTINUE READING
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