Comparative study of phagocytosis and intracellular bactericidal activity of human monocytes and polymorphonuclear cells

@article{Pruzanski1988ComparativeSO,
  title={Comparative study of phagocytosis and intracellular bactericidal activity of human monocytes and polymorphonuclear cells},
  author={Waldemar Pruzanski and Sachie Saito},
  journal={Inflammation},
  year={1988},
  volume={12},
  pages={87-97}
}
Simultaneous assessment of the total number of bacteria (TNB) ingested, phagocytosis (Ph), phagocytic index (PI), and intracellular bactericidal activity (ICBA) of human monocytes was done by applying the fluorochrome acridine orange technique. Living bacteria stained orthochromatically green, whereas the dead ones were metachromatically red. The stain of extracellular bacteria was completely quenched by crystal violet counterstain. Using the Hypaque-Ficoll separation method combined with glass… CONTINUE READING

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