Comparative study of mitotic chromosomes in two blowflies, Lucilia sericata and L. cluvia (Diptera, Calliphoridae), by C- and G-like banding patterns and rRNA loci, and implications for karyotype evolution

  title={Comparative study of mitotic chromosomes in two blowflies, Lucilia sericata and L. cluvia (Diptera, Calliphoridae), by C- and G-like banding patterns and rRNA loci, and implications for karyotype evolution},
  author={M. Chirino and L. Rossi and M. J. Bressa and J. P. Luaces and M. S. Merani},
  journal={Comparative Cytogenetics},
  pages={103 - 118}
Abstract The karyotypes of Lucilia cluvia (Walker, 1849) and Lucilia sericata (Meigen, 1826) from Argentina were characterized using conventional staining and the C- and G-like banding techniques. Besides, nucleolus organizer regions (NORs) were detected by fluorescent in situ hybridization (FISH) and silver staining technique. The chromosome complement of these species comprises five pairs of autosomes and a pair of sex chromosomes (XX/XY, female/male). The autosomes of both species have the… Expand
Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects
The cytogenetic observations on L. sericata indicate that the accumulation of heterochromatin in the genome could be involved in chromosomal divergence and karyotype evolution of this species, as demonstrated in other Diptera. Expand
Karyotypic features including organizations of the 5S, 45S rDNA loci and telomeres of Scadoxus multiflorus (Amaryllidaceae)
It is shown that the ends of Scadoxus multiflorus chromosomes harbor human instead of Arabidopsis-type telomere sequences, which is important for better understanding of evolution in Amaryllidaceae. Expand
Analysis of the Metaphase Chromosome Karyotypes in Imaginal Discs of Aedes communis, Ae. punctor, Ae. intrudens, and Ae. rossicus (Diptera: Culicidae) Mosquitoes
In this study, cytogenetic analysis of the metaphase chromosomes from imaginal discs of Aedes (Diptera: Culicidae) mosquitoes—Aedes communis, Ae. punctor, Ae. intrudens, and Ae. rossicus—wasExpand
A Comparative Analysis of the Metaphase Karyotypes of Aedes excrucians, Ae. behningi, and Ae. euedes (Diptera: Culicidae) Imaginal Discs
ABSTRACT: Karyotypes of Aedes (Culicidae) mosquitoes (Ae. excrucians, Ae. behningi, and Ae. euedes) have been analyzed using the metaphase chromosomes of imaginal discs. Lacto-aceto-orcein,Expand
Comparison of different cytogenetic methods and tissue suitability for the study of chromosomes in Cimex lectularius (Heteroptera, Cimicidae)
The pros and cons of the spreading method compared with the traditional squashing method are sought to show and gonad, midgut and embryo tissue in Cimex lectularius Linnaeus, 1758 chromosome research and production of figures of whole mitosis and meiosis are discussed. Expand
The genomes of a monogenic fly: views of primitive sex chromosomes
The genomes of Chrysomya rufifacies, a monogenic blow fly, are presented by separately sequencing and assembling each type of female and the male to provide new clues regarding the origination and evolution of the diverse sex-d determining mechanisms observed within Diptera. Expand


Cytogenetic studies of Calliphora vicina and Lucilia sericata (Diptera: Calliphoridae) from northwestern Egypt.
  • G. M. El-Bassiony
  • Biology, Medicine
  • Journal of the Egyptian Society of Parasitology
  • 2006
The blow fly, Calliphora vicina and the green bottle fly, Lucilia sericata were collected from the north coast of Egypt and the karyotype of both flies was characterized by cytotaxonomical methods, including chromosome number, shape, relative length, arm ratio, centromeric index and designation. Expand
C-banding and FISH in chromosomes of the blow flies Chrysomya megacephala and Chrysomya putoria (Diptera, Calliphoridae).
Results confirm that C. putoria was the species introduced into Brazil in 1970s, and not C. chloropyga as formerly described, and there are no substantial differences in the karyotype morphology of these two species. Expand
Comparison of the mitotic karyotypes of Ceratitis capitata, Ceratitis rosa, and Trirhithrum coffeae (Diptera: Tephritidae) by C-banding and FISH.
The sex chromosomes of the tephritid fruit fly Ceratitis capitata are heteromorphic and it is shown that only 15% of the Y chromosome is required for male determination and male fertility, and Y-chromosomal length polymorphism is expected in natural populations. Expand
Karyotypes, constitutive heterochromatin, and genomic DNA values in the blowfly genera Chrysomya, Lucilia, and Protophormia (Diptera: Calliphoridae).
The data suggest that the interspecific DNA differences in most species are mainly due to quantitative variation of (repetitive) sequences lying outside the centromeric heterochromatin blocks of the large chromosomes. Expand
C, Q and H-banding in the analysis of Y chromosome rearrangements in Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae)
In Lucilia cuprina C-banding produces procentric bands on all autosomes and deep staining over most of the X and Y chromosomes which conciderably facilitates the analysis of complex Y chromosomeExpand
Codling moth cytogenetics: karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes.
The demonstrated ways of W chromosome identification will facilitate the development of genetic sexing strains desirable for pest control using the sterile insect technique. Expand
Sex Chromosome Evolution in Cotton Stainers of the Genus Dysdercus (Heteroptera: Pyrrhocoridae)
The hypothesis that the neo-X chromosome evolved by insertion of the original X chromosome into 1 NOR-bearing autosome in an ancestor carrying the X0 system is supported, as a consequence, the homologue of this NOR-autosome became the Neo-Y chromosome. Expand
Karyotype of the bird blowfly, Protocalliphora falcozi Séguy, 1928 (Diptera, Calliphoridae).
The karyotype structure of Protocalliphora falcozi is described for the first time and shows a pericentromeric position of constitutive heterochromatin in all autosomes. Expand
Fluorescence in situ hybridization of ribosomal DNA to mitotic chromosomes of tsetse flies (Diptera: Glossinidae: Glossina)
The rDNA genes are the first physically mapped markers in tsetse flies and will be useful for mapping approaches, and the C-banding pattern obtained by in situ hybridization was compared with Giemsa C- banding patterns that were published previously. Expand
Cytological characterisation of heterochromatin in mitotic and meiotic chromosomes of the Old World screwworm fly, Chrysomya bezziana (Diptera: Calliphoridae).
Mitotic and meiotic chromosomes of the Old World screwworm fly were studied using C-banding and quinacrine and counterstain-enhanced fluorescence techniques, indicating that much of the heterochromatin in the sex chromosomes has unusual structural properties. Expand