Comparative study of methods for extraction and purification of environmental DNA from soil and sludge samples.

@article{Roh2006ComparativeSO,
  title={Comparative study of methods for extraction and purification of environmental DNA from soil and sludge samples.},
  author={Changhyun Roh and Francois Villatte and Byung-Gee Kim and Rolf D. Schmid},
  journal={Applied biochemistry and biotechnology},
  year={2006},
  volume={134 2},
  pages={
          97-112
        }
}
An important prerequisite for successful construction of a metagenome library is an efficient procedure for extracting DNA from environmental samples. We compared three indirect and four direct extraction methods, including a commercial kit, in terms of DNA yield, purity, and time requirement. A special focus was on methods that are appropriate for the extraction of environmental DNA (eDNA) from very limited sample sizes (0.1 g) to enable a highly parallel approach. Direct extraction procedures… CONTINUE READING
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