Comparative study of freeze-substitution techniques for X-ray microanalysis of biological tissue.

@article{Plsgrd1994ComparativeSO,
  title={Comparative study of freeze-substitution techniques for X-ray microanalysis of biological tissue.},
  author={E P{\aa}lsg{\aa}rd and Ulf Lindh and Godfried M. Roomans},
  journal={Microscopy research and technique},
  year={1994},
  volume={28 3},
  pages={254-8}
}
A comparative study of some aspects of freeze-substitution as a preparative method for X-ray microanalysis of diffusible ions in biological specimens was carried out. Four substitution fluids were compared with each other and with cryosections. As criteria, elemental ratios as determined by X-ray microanalysis of thin sections of mouse pancreas were used. Freeze-substitution for 3 weeks in diethyl ether or for 2 days in tetrahydrofurane gave results comparable to those obtained in cryosections… CONTINUE READING

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