Comparative study of five different DNA fingerprint techniques for molecular typing of Streptococcus pneumoniae strains.

@article{Hermans1995ComparativeSO,
  title={Comparative study of five different DNA fingerprint techniques for molecular typing of Streptococcus pneumoniae strains.},
  author={Peter Hermans and Marcel Sluijter and Theo Hoogenboezem and Herre Heersma and Alex van Belkum and Ronald de Groot},
  journal={Journal of clinical microbiology},
  year={1995},
  volume={33 6},
  pages={1606-12}
}
The aim of this study was to identify the strengths and weaknesses of five DNA fingerprint methods for epidemiological typing of Streptococcus pneumoniae. We investigated the usefulness of (i) ribotyping, (ii) BOX fingerprinting with the BOX repetitive sequence of S. pneumoniae as a DNA probe, (iii) PCR fingerprinting with a primer homologous to the enterobacterial repetitive intergenic consensus sequence, (iv) pulsed-field gel electrophoresis of large DNA fragments, and (v) restriction… CONTINUE READING

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Molecular cloning: a laboratory manual, 2nd ed

  • J. Sambrook, E. F. Fritsch, T. Maniatis
  • 1989
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