Comparative studies on the sensitivity of polymerase chain reaction and microscopic examination for the detection of Trypanosoma evansi in experimentally infected mice.

@article{Ijaz1998ComparativeSO,
  title={Comparative studies on the sensitivity of polymerase chain reaction and microscopic examination for the detection of Trypanosoma evansi in experimentally infected mice.},
  author={M Khalid Ijaz and M. S. A. Nur-e-Kamal and Amr Mohamed and Fazal Karim Dar},
  journal={Comparative immunology, microbiology and infectious diseases},
  year={1998},
  volume={21 3},
  pages={
          215-23
        }
}

Comparative studies on the sensitivity of polymerase chain reaction (PCR) and microscopic examination for the detection of Trypanosoma evansi in horses

TLDR
PCR is a useful diagnostic tool for detecting Trypanosoma evansi infected horses in the very early stages where microscopic examination is equivocal, and field application of PCR is clearly indicated by the results.

PCR-ELISA for diagnosis of Trypanosoma evansi in animals and vector.

TLDR
The PCR-ELISA was shown to detect 33 samples of T. evansi infected blood of animals and 10 mosquitoes from different geographical area in Thailand, implying that the technique of PCR- ELISA is not only beneficial for diagnosis of the parasite but also useful for epidemiological study and designing rational trypanosomiasis control program.

Comparative evaluation of parasitological, serological and DNA amplification methods for diagnosis of natural trypanosomal infection in equines.

TLDR
PCR test was found to be a superior test over MHCT and ELISA for the diagnosis of trypanosome in equines and can be used in field conditions.

EVALUATION OF THE CARD AGGLUTINATION TEST (CATT/T. EVANSI) IN COMPARISON WITH PCR FOR DETECTION OF TRYPANOSOMA EVANSI INFECTION IN DONKEYS IN EGYPT

TLDR
Despite of the simplicity and low-coast of CATT test, it is recommended to use it in combination with PCR technique for accurate and reliable results.

Development of a loop-mediated isothermal amplification assay based on RoTat1.2 gene for detection of Trypanosoma evansi in domesticated animals.

TLDR
The current LAMP assay has appealing point of care characteristics to visually monitor the results, lessen the need of post DNA amplification procedure, and enable this method to be applied as a rapid and sensitive molecular diagnostic tool in under resourced laboratories and field setup.

References

SHOWING 1-10 OF 13 REFERENCES

Detection of circulating trypanosomal antigens in Trypanosoma evansi-infected animals using a T. brucei group-specific monoclonal antibody.

  • V. NantulyaE. Bajyana SongaR. Hamers
  • Biology
    Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit
  • 1989
An antigen-detection enzyme immunoassay based on a T. brucei group-specific monoclonal antibody was used for the detection of circulating antigens in several animal species experimentally infected

Polymerase chain reaction-based detection of Trypanosoma cruzi DNA in serum

DNAs prepared from chagasic patients' sera were amplified by the polymerase chain reaction using oligonucleotide primers which anneal specifically to a highly repetitive sequence of Trypanosoma cruzi

Detection of Trypanosoma congolense and Trypanosoma brucei subspecies by DNA amplification using the polymerase chain reaction

TLDR
PCR amplification is ideally suited for large-scale studies on the prevalence of African trypanosomes in both mammalian blood and insect vectors as it is about 100 times more sensitive than previous detection methods based on radioactive DNA probes.

Trypanosoma evansi in Asia.

The polymerase chain reaction: amplifying our options.

  • J. Peter
  • Medicine
    Reviews of infectious diseases
  • 1991
TLDR
The application of PCR in four contexts is presented along with a discussion of the potential of this technique to elucidate the natural history of infectious disease and to serve as a tool for the molecular elucidation of acute and chronic illness.

The haematocrit centrifuge for the detection of trypanosomes in blood.

A capillary tube holder, devised to minimize shadow effects during microscopic examination of haematocrit capillary tubes for trypanosomes, is described. A blood dispenser, designed to remove