Comparative studies on the interaction of benzo[a]pyrene with cells derived from poikilothermic and homeothermic vertebrates. I. Metabolism of benzo[a]pyrene.

Abstract

The metabolism of benzo[a]pyrene (BP) to water-soluble derivatives was measured in cell cultures derived from poikilothermic and homeothermic vertebrates. Tritiated BP was added to the cultures and, after 24 hours of incubation, the medium was extracted with chloroform:methanol:water. The amount of BP metabolized per cell (relative efficiency of metabolism… (More)

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