Comparative studies on platelet membrane preparations.

For the isolation of platelet plasma and cytoplasmatic membranes, the following methods were applied. 1. Glycerol-osmotic lysis technique, combined with sonication. 2. Glycerol-osmotic lysis technique carried out on fresh or frozen platelets. 3. Detergent treatment followed by separation of the protein fractions insoluble at low ionic strength. The… (More)