Comparative studies between in vivo and in vitro spermatogenesis of Japanese eel (Anguilla japonica).

@article{Miura2002ComparativeSB,
  title={Comparative studies between in vivo and in vitro spermatogenesis of Japanese eel (Anguilla japonica).},
  author={Takeshi Miura and Nobutaka Ando and Chiemi I. Miura and Kohei Yamauchi},
  journal={Zoological science},
  year={2002},
  volume={19 3},
  pages={321-9}
}
In order to check the quality of in vitro spermatogenesis of Japanese eel, in vitrol 1-ketotestosterone (11-KT) induced spermatogenesis was compared with in vivo spermatogenesis induced by a single injection of human chorionic gonadotropin (hCG) in detail. DNA contents of germ cells from in vitro and in vivo testicular fragments were compared using flow cytometry. Since the in vitro result of flow cytometry showed prominent 1C peak including spermatozoa and spermatids, the reduction of DNA by… CONTINUE READING