Comparative shotgun proteomic profiles for identification of differentially expressed proteins in response to topping in Nicotiana tabacum leaves

@article{Yang2016ComparativeSP,
  title={Comparative shotgun proteomic profiles for identification of differentially expressed proteins in response to topping in Nicotiana tabacum leaves},
  author={Huijuan Yang and Fei Su and Jing Wang and Hongzhi Shi},
  journal={Acta Physiologiae Plantarum},
  year={2016},
  volume={39},
  pages={1-10}
}
The aim of this study was to explore the differences of protein expression in tobacco leaves in response to topping. Comparative shotgun proteomic approach combined with mass spectrometry was used to screen the differentially expressed proteins between leaves before topping and leaves after topping. Additionally, quantitative real-time PCR was applied to validate the differentially expressed proteins. A total of 198 and 193 differentially expressed proteins were identified in leaves before and… CONTINUE READING