Comparative sequence analyses of sixteen reptilian paramyxoviruses.

  title={Comparative sequence analyses of sixteen reptilian paramyxoviruses.},
  author={Winfried Ahne and William N. Batts and Gael Kurath and James R. Winton},
  journal={Virus research},
  volume={63 1-2},
Viral genomic RNA of Fer-de-Lance virus (FDLV), a paramyxovirus highly pathogenic for reptiles, was reverse transcribed and cloned. Plasmids with significant sequence similarities to the hemagglutinin-neuraminidase (HN) and polymerase (L) genes of mammalian paramyxoviruses were identified by BLAST search. Partial sequences of the FDLV genes were used to design primers for amplification by nested polymerase chain reaction (PCR) and sequencing of 518-bp L gene and 352-bp HN gene fragments from a… CONTINUE READING


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