Comparative proteomic and metabolomic analysis of Streptomyces tsukubaensis reveals the metabolic mechanism of FK506 overproduction by feeding soybean oil

@article{Wang2017ComparativePA,
  title={Comparative proteomic and metabolomic analysis of Streptomyces tsukubaensis reveals the metabolic mechanism of FK506 overproduction by feeding soybean oil},
  author={Jun Wang and Huanhuan Liu and Di Huang and Lina Jin and Cheng Wang and Jianping Wen},
  journal={Applied Microbiology and Biotechnology},
  year={2017},
  volume={101},
  pages={2447-2465}
}
FK506 (tacrolimus) is a 23-membered polyketide macrolide that possesses powerful immunosuppressant activity. In this study, feeding soybean oil into the fermentation culture of Streptomyces tsukubaensis improved FK506 production by 88.8%. To decipher the overproduction mechanism, comparative proteomic and metabolomic analysis was carried out. A total of 72 protein spots with differential expression in the two-dimensional gel electrophoresis (2-DE) were identified by matrix-assisted laser… CONTINUE READING