Comparative prognostic value of HPV16 E6 mRNA compared with in situ hybridization for human oropharyngeal squamous carcinoma.

@article{Shi2009ComparativePV,
  title={Comparative prognostic value of HPV16 E6 mRNA compared with in situ hybridization for human oropharyngeal squamous carcinoma.},
  author={Wei Shi and Hisayuki Kato and Bayardo Perez-Ord{\'o}{\~n}ez and Melania Pintilie and Shaohui Huang and Angela M Y Hui and Brian O'Sullivan and John Waldron and Bernard Cummings and John Kim and Jolie Ringash and Laura Ann Dawson and Patrick Gullane and Lillian L. Siu and Maura L. Gillison and Fei-fei Liu},
  journal={Journal of clinical oncology : official journal of the American Society of Clinical Oncology},
  year={2009},
  volume={27 36},
  pages={6213-21}
}
PURPOSE A significant proportion of oropharyngeal squamous cell carcinomas (OSCC) are associated with the human papilloma virus (HPV), particularly HPV16. The optimal method for HPV determination on archival materials however, remains unclear. We compared a quantitative real-time polymerase chain reaction (qRT-PCR) assay for HPV16 mRNA to a DNA in situ hybridization (ISH) method, and evaluated their significance for overall (OS) and disease-free (DFS) survival. PATIENTS AND METHODS Matched… CONTINUE READING