Comparative genomics and site-directed mutagenesis support the existence of only one input channel for protons in the C-family (cbb3 oxidase) of heme-copper oxygen reductases.

@article{Hemp2007ComparativeGA,
  title={Comparative genomics and site-directed mutagenesis support the existence of only one input channel for protons in the C-family (cbb3 oxidase) of heme-copper oxygen reductases.},
  author={James Hemp and Huazhi Han and Jung Hyeob Roh and S. Kaplan and Todd J. Mart{\'i}nez and Robert B Gennis},
  journal={Biochemistry},
  year={2007},
  volume={46 35},
  pages={9963-72}
}
Oxygen reductase members of the heme-copper superfamily are terminal respiratory oxidases in mitochondria and many aerobic bacteria and archaea, coupling the reduction of molecular oxygen to water to the translocation of protons across the plasma membrane. The protons required for catalysis and pumping in the oxygen reductases are derived from the cytoplasmic side of the membrane, transferred via proton-conducting channels comprised of hydrogen bond chains containing internal water molecules… CONTINUE READING