Comparative genomic hybridization analysis of human oocytes and polar bodies.

@article{Fragouli2006ComparativeGH,
  title={Comparative genomic hybridization analysis of human oocytes and polar bodies.},
  author={Elpida Fragouli and Dagan Wells and Alan D Thornhill and Paul Serhal and Michael J. W. Faed and Joyce Catherine Harper and Joy D. A. Delhanty},
  journal={Human reproduction},
  year={2006},
  volume={21 9},
  pages={2319-28}
}
BACKGROUND Classical cytogenetic methods and fluorescent in situ hybridization (FISH) have been employed for the analysis of chromosomal abnormalities in human oocytes. However, these methods are limited by the need to spread the sample on a microscope slide, a process that risks artefactual chromosome loss. Comparative genomic hybridization (CGH) is a DNA-based method that enables the investigation of the entire chromosome complement. We optimized and evaluated a CGH protocol for the… CONTINUE READING