Comparative flow cytometric study of clonal excess in leukaemic peripheral blood from patients suffering from chronic lymphocytic leukaemia (B-CLL) by different antibodies, staining techniques and the effects of blood storage.

Abstract

This investigation studied the effects of cell preparation methods, different antibody panels and blood storage on antigen expression of abnormal B lymphocytes from patients with B-CLL. Blood specimens collected in Heparin de novo were processed by using conventional Hypaque-Ficoll density gradient centrifugation and whole blood lysis. These were stored for… (More)

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@article{Schmidtke1999ComparativeFC, title={Comparative flow cytometric study of clonal excess in leukaemic peripheral blood from patients suffering from chronic lymphocytic leukaemia (B-CLL) by different antibodies, staining techniques and the effects of blood storage.}, author={Gunter Schmidtke and Ulli Schmucker and Guenter Brittinger and H G H{\"o}ffkes}, journal={Clinical and laboratory haematology}, year={1999}, volume={21 2}, pages={103-12} }