Comparative expression and purification of human glutamic acid decarboxylase from Saccharomyces cerevisiae and Pichia pastoris.

Abstract

The yeast cell factory is a potentially useful source of proteins in general. They include glutamic acid decarboxylase (GAD), which is one of the major autoantigens for Type 1 diabetes. We have created a hybrid form of GAD consisting of amino acids 1-101 of the human GAD67 protein fused to amino acids 96-585 of the human GAD65 protein, and have modified… (More)

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Cite this paper

@article{Papakonstantinou2000ComparativeEA, title={Comparative expression and purification of human glutamic acid decarboxylase from Saccharomyces cerevisiae and Pichia pastoris.}, author={Papakonstantinou and D Law and Gardiner and Rowley and Mackay}, journal={Enzyme and microbial technology}, year={2000}, volume={26 9-10}, pages={645-652} }