[Comparative evaluation of methods of obtaining phagolysates modelled on d'Herelle's plague microbe phage].

Abstract

Various methods for phage multiplication have been tested: the method of cellophane membranes, the method of combined media, the method of forced aeration of a liquid medium, growth at suboptimal temperature and growth at optimal temperature under stationary conditions. The phage in a high concentration (n X 10(10) plaque-forming units per ml) can be obtained by any of these methods with the exception of the last one yielding lower (by 1 or 2 orders) effectiveness of multiplication. The main factors which determine the titer of the phage in lysate are concentration of live cells in the suspension and temperature of growth.

Cite this paper

@article{Marchenkov1982ComparativeEO, title={[Comparative evaluation of methods of obtaining phagolysates modelled on d'Herelle's plague microbe phage].}, author={V I Marchenkov and I M Alutin and A V Suichmezova}, journal={Zhurnal mikrobiologii, epidemiologii, i immunobiologii}, year={1982}, volume={11}, pages={64-7} }