Comparative drug metabolism of diazepam in hepatocytes isolated from man, rat, monkey and dog.

@article{Seddon1989ComparativeDM,
  title={Comparative drug metabolism of diazepam in hepatocytes isolated from man, rat, monkey and dog.},
  author={T Seddon and I Michelle and Richard J. Chenery},
  journal={Biochemical pharmacology},
  year={1989},
  volume={38 10},
  pages={1657-65}
}
Diazepam (DZ) was used as a substrate in drug metabolism studies to characterise the differences in metabolite profiles in hepatocytes isolated from four species: Wistar rat, cynomolgus monkey, beagle dog and man. Hepatocytes were incubated with DZ (20 microM) for 180 min at 3 hr post isolation in culture, and the disappearance of parent compound and appearance of its metabolites determined. DZ disappearance was found to be monoexponential in rat, monkey and human cells, but that DZ… CONTINUE READING

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