Comparative cross-linking study on the 50S ribosomal subunit from Escherichia coli.

@article{Walleczek1989ComparativeCS,
  title={Comparative cross-linking study on the 50S ribosomal subunit from Escherichia coli.},
  author={Jan Walleczek and Til Martin and Bernhard Redl and Marina Stoeffler-Meilicke and Georg St{\"o}ffler},
  journal={Biochemistry},
  year={1989},
  volume={28 9},
  pages={
          4099-105
        }
}
We have carried out an extensive protein-protein cross-linking study on the 50S ribosomal subunit of Escherichia coli using four different cross-linking reagents of varying length and specificity. For the unambiguous identification of the members of the cross-linked protein complexes, immunoblotting techniques using antisera specific for each individual ribosomal protein have been used, and for each cross-link, the cross-linking yield has been determined. With the smallest cross-linking reagent… CONTINUE READING
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