Comparative characterization of mAb producing hapten-specific hybridoma cells by flow cytometric analysis and ELISA.


A novel method that optimizes the screening for antibody-secreting hapten-specific hybridoma cells by using flow cytometry is described. Cell clones specific for five different haptens were analyzed. We selectively double stained and analyzed fixed hybridoma cells with fluorophore-labeled haptens to demonstrate the target-selectivity, and with a fluorophore… (More)
DOI: 10.1016/j.jim.2014.07.004


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