Comparative calcium binding of leucine-rich amelogenin peptide and full-length amelogenin.

@article{Le2006ComparativeCB,
  title={Comparative calcium binding of leucine-rich amelogenin peptide and full-length amelogenin.},
  author={Thuan Q Le and Miriam Gochin and John D. B. Featherstone and Wu Li and Pamela K Denbesten},
  journal={European journal of oral sciences},
  year={2006},
  volume={114 Suppl 1},
  pages={320-6; discussion 327-9, 382}
}
Leucine-rich amelogenin peptide (LRAP) is an alternately spliced amelogenin. LRAP is known to bind to hydroxyapatite, and has been shown to signal mesenchymal cells to proliferate, but its function in enamel formation is unclear. The purpose of this study was to determine the calcium-binding properties and structure of recombinant human LRAP (rLRAP) compared with full-length amelogenin (rH174). rLRAP and rH174 were synthesized in Escherichia coli and purified by affinity chromatography and… CONTINUE READING