Comparative analysis of the localization of lipoteichoic acid in Streptococcus agalactiae and Streptococcus pyogenes

@article{Mattingly1987ComparativeAO,
  title={Comparative analysis of the localization of lipoteichoic acid in Streptococcus agalactiae and Streptococcus pyogenes},
  author={S. Mattingly and B. Johnston},
  journal={Infection and Immunity},
  year={1987},
  volume={55},
  pages={2383 - 2386}
}
The cellular locations of deacylated lipoteichoic acid (dLTA) and lipoteichoic acid (LTA) were examined in late-exponential-phase cells of a serotype III strain of Streptococcus agalactiae (group B streptococci [GBS]) isolated from an infant with late-onset meningitis and compared with a fresh clinical isolate of Streptococcus pyogenes (group A streptococci [GAS]). LTA and dLTA were found to be associated with the protoplast membranes of both organisms, with only dLTA found in mutanolysin cell… Expand
Molecular analysis of lipoteichoic acid from Streptococcus agalactiae
TLDR
This procedure was used to examine the effect of penicillin and chloramphenicol on the synthesis, turnover, and heterogeneity of LTA in S. agalactiae and determined that LTA was released as a consequence of deacylation. Expand
Electron microscopic localization of lipoteichoic acid on group A streptococci.
  • M. Rýc, B. Wagner, M. Wagner, R. Bicova
  • Biology, Medicine
  • Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology
  • 1988
The location of lipoteichoic acid (LTA) on the surface of group A streptococci was studied by immunoelectron microscopic and ultrastructural cytochemical methods, i.e. by means of LTA antibodiesExpand
Adhesion of glucosyltransferase phase variants to Streptococcus gordonii bacterium-glucan substrata may involve lipoteichoic acid
TLDR
In vitro findings suggest that, in vivo, sucrose and lipoteichoic acid may modify dental plaque development by enhancing or inhibiting the attachment of additional bacteria. Expand
Bioinformatic insights into the biosynthesis of the Group B carbohydrate in Streptococcus agalactiae.
TLDR
Genomic analysis has allowed us to predict the pathway leading to the biosynthesis of GBC of S. agalactiae, and analysis of the sugar linkages present in the Group B carbohydrate structure suggests that GBC is the major teichoic acid-like polymer in the cell wall of S., whereas lipoteichoic Acid is the dominant poly(glycerophosphate) antigen. Expand
In vitro method to differentiate isolates of type III Streptococcus agalactiae from symptomatic and asymptomatic patients
TLDR
The results suggest that the physiological growth response of clinical isolates of group B streptococci to phosphate can serve as a diagnostic aid in screening potentially virulent strains in pregnant women and newborn infants. Expand
Roles of the bacterial cell wall and capsule in induction of tumor necrosis factor alpha by type III group B streptococci
TLDR
Findings indicate that GB-PS and PG are the bacterial cell wall components primarily evoking TNF-alpha release, which, alone or in concert with other factors, may be responsible for septic shock accompanying GBS sepsis. Expand
Gram-Positive Pyogenic Cocci
TLDR
There is a growing body of evidence to suggest that all of the pyogenic Gram-positive cocci rely on multiple adhesins in order to adhere avidly to substrata. Expand
Cloning and functional analysis of the P97 swine cilium adhesin gene of Mycoplasma hyopneumoniae
TLDR
Both recombinant P97 protein expressed in an Escherichia coli opal suppressor host and M. hyopneumoniae bound specifically to swine cilia, and the binding was inhibited by heparin and fucoidan, thus supporting the hypothesis that P97 was actively involved in binding to swining cilia in vivo. Expand
Blood-brain barrier invasion by group B Streptococcus depends upon proper cell-surface anchoring of lipoteichoic acid.
TLDR
It is suggested that LTA expression on the GBS surface plays a role in bacterial interaction with BBB endothelium and the pathogenesis of neonatal meningitis. Expand
Group B Streptococcal β-Hemolysin Promotes Injury of Lung Microvascular Endothelial Cells
TLDR
It is concluded that GBS β-hemolysin promotes LMvEC injury and increases permeability in vitro, and speculate that G BS β- hemolysin contributes to the pathogenesis of alveolar edema and hemorrhage in early onset GBS pneumonia. Expand
...
1
2
...

References

SHOWING 1-10 OF 15 REFERENCES
Kinetic and chemical analyses of the biologic significance of lipoteichoic acids in mediating adherence of serotype III group B streptococci
TLDR
LTA appears to enhance the ability of virulent group B streptococci to bind to HEC and HFC with stronger avidity by virtue of the increased length of the cell-associated polymers synthesized by these strains. Expand
Role of cellular lipoteichoic acids in mediating adherence of serotype III strains of group B streptococci to human embryonic, fetal, and adult epithelial cells
TLDR
Results indicate that possible receptor sites for LTA present on cells in prenatal stages of development may differ from those of adult cells, which may result in increased susceptibility of newborn infants to group B streptococcal disease. Expand
Association of type- and group-specific antigens with the cell wall of serotype III group B streptococcus
TLDR
The covalent association of TSA, the group B polysaccharide, and the peptidoglycan was demonstrated by the presence of N-acetylmuramic acid, rhamnose, alanine, glutamate, and lysine in mutanolysin-extracted TSA material purified by DEAE-Sephacel anion exchange and Sepharose 4B gel chromatography. Expand
Formation of Molecular Complexes Between a Structurally Defined M Protein and Acylated or Deacylated Lipoteichoic Acid of Streptococcus pyogenes
TLDR
Results indicate that the LTA molecule is able to bind via its polyanionic backbone to positively charged residues of surface proteins of cells of S. pyogenes. Expand
Comparative studies of lipoteichoic acids from several Bacillus strains
TLDR
Structural studies were carried out on lipoteichoic acids obtained from defatted cells of 10 Bacillus strains by phenol-water partition followed by chromatography on DEAE-Sephacel and Octyl-Sepharose columns, and results indicate that in the lipote Jerichoic acids of group A strains the polymer chains are believed to be directly linked to the acylglycerol anchors. Expand
Growth and Amino Acid Requirements of Various Strains of Group B Streptococci
TLDR
The group B streptococci, with minor exceptions, were very homogeneous in their amino acid requirements under both aerobic and anaerobic growth conditions. Expand
Excretion of lipoteichoic acid by group A streptococci. Influence of penicillin on excretion and loss of ability to adhere to human oral mucosal cells.
TLDR
Streptococci that were stimulated with penicillin to lose their lipoteichoic acid lost their ability to adhere to buccal mucosal cells, suggesting thatPenicillin may influence bacterial ecology by mechanisms other than killing sensitive organisms. Expand
Biosynthesis of cell wall peptidoglycan and polysaccharide antigens by protoplasts of type III group B Streptococcus
TLDR
The formation of a nascent peptidoglycan-group-specific antigen of type III group B Streptococcus at the cell membrane level was demonstrated with an M-1 mutanolysin-prepared protoplast system, suggesting that a more complex cell wall matrix may be necessary before linkage of the high-molecular-weight antigen takes place. Expand
Release of lipoteichoic acid from Streptococcus sanguis: stimulation of release during penicillin treatment
TLDR
Penicillin-treated cells released the bulk of water-soluble, glycerol-labeled polymers, and they were not replenished by synthesis during antibiotic treatment, and a major portion of the extracellular polymers was characterized as acylated lipoteichoic acid. Expand
The adhesion of human and bovine isolates of Streptococcus agalactiae (group B) to bovine mammary gland epithelial cells.
TLDR
Electron microscopy showed that pepsin treatment removed a fimbrial layer from the bacterial surface in S. agalactiae strains that adhered to mammary gland epithelial cells in vitro. Expand
...
1
2
...