Comparative analysis of the effects of ultrasound-targeted microbubble destruction on recombinant adeno-associated virus- and plasmid-mediated transgene expression in human retinal pigment epithelium cells.

@article{Zheng2009ComparativeAO,
  title={Comparative analysis of the effects of ultrasound-targeted microbubble destruction on recombinant adeno-associated virus- and plasmid-mediated transgene expression in human retinal pigment epithelium cells.},
  author={Xiao-zhi Zheng and Ying Wu and Hongli Li and Lian-fang Du and Hui-ping Wang and Qing Gu},
  journal={Molecular medicine reports},
  year={2009},
  volume={2 6},
  pages={
          937-42
        }
}
Ultrasound-targeted microbubble destruction (UTMD) has been utilized to deliver a drug/gene into cells in both in vitro and in vivo studies. This study was performed to investigate the feasibility of UTMD-enhanced recombinant adeno-associated virus- (rAAV) and plasmid-mediated transfection into the human retinal pigment epithelium (RPE) cell line ARPE-19. Additionally, the transfection efficiency of rAAV and plasmid was compared in order to choose the appropriate gene vector or strategy to be… 
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Recombinant adeno-associated virus-, polyethylenimine/plasmid- and lipofectamine/carboxyfluorescein-labeled small interfering RNA-based transfection in retinal pigment epithelial cells with ultrasound and/or SonoVue.
TLDR
UTMD/US may be of use in enhancing rAAV, PEI/pDNA and L/siRNA transgene expression of ARPE‑19 cells in vitro and may provide useful information to guide future in vivo and transfection studies.
Enhanced downregulation of transforming growth factor‑β2 in rat retinal pigment epithelium cells by adeno‑associated virus‑mediated ribonucleic acid interference combined with ultrasound or microbubbles.
TLDR
Under the optimal conditions of rAAv delivery, enhanced TGFβ2 gene silencing with a combination of US or SonoVue with rAAV2‑TGFβ 2 shRNA resulted in a significant decrease in mRNA levels compared with r AAV2‐TGF β2 shRNA alone.
Recombinant adenovirus‐mediated human telomerase reverse transcriptase gene can stimulate cell proliferation and maintain primitive characteristics in bovine mammary gland epithelial cells
TLDR
The adenovirus‐mediated hTERT gene could not only extend the cell lifespan, but also maintained the primary characteristics of the cells, suggesting it may be possible to extend the use of a wide variety of non‐human mammalian cells in this way.
Ultrasound-targeted microbubble destruction promotes proliferative vitreoretinopathy induced by RPE-J cells and platelet-rich plasma
TLDR
UTMD promotes PVR induced by RPE-J cells and PRP which provide a new method for the development of rat PVR model, and the effects of UTMD should be taken into consideration when using UTMD as an approach to attenuate PVR.
Gene therapy for ocular diseases meditated by ultrasound and microbubbles (Review)
TLDR
Ultrasound-targeted microbubble destruction (UTMD), with the advantages of high safety, repetitive applicability and tissue targeting, has become a potential strategy for gene- and drug delivery and the current status of gene therapy of ocular diseases is discussed.
Entry of Oncolytic Herpes Simplex Virus into Human Squamous Cell Carcinoma Cells by Ultrasound
TLDR
It is suggested that oncolytic HSV-1 RH2 can be introduced into SAS cells through ultrasound-mediated pores of the cell membrane that are resealed after an interval when the interval from ultrasound exposure to virus inoculation was prolonged.
A novel approach to attenuate proliferative vitreoretinopathy using ultrasound‐targeted microbubble destruction and recombinant adeno‐associated virus‐mediated RNA interference targeting transforming growth factor‐β2 and platelet‐derived growth factor‐B
TLDR
The present study aimed to investigate the feasibility of the attenuation of PVR by a combinatorial use of UTMD and recombinant adeno‐associated virus and RNAi targeting transforming growth factor (TGF)‐β2 and platelet‐derived growth factor(B)‐B.

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