Comparative analysis of proliferative potential and clonogenicity of MACS-immunomagnetic isolated CD34+ and CD133+ blood stem cells derived from a single donor.

@article{Freund2006ComparativeAO,
  title={Comparative analysis of proliferative potential and clonogenicity of MACS-immunomagnetic isolated CD34+ and CD133+ blood stem cells derived from a single donor.},
  author={Daniel Freund and John Oswald and Silvia Feldmann and Gerhard Ehninger and Denis Corbeil and Martin Bornhaeuser},
  journal={Cell proliferation},
  year={2006},
  volume={39 4},
  pages={325-32}
}
A novel stem cell marker prominin-1 (CD133) has been shown to be expressed on a subpopulation of CD34(+) haematopoietic stem and progenitor cells. The aim of this study was to compare in parallel commercially available CD34(+) and CD133(+) isolation methods based on paramagnetic bead-coupled antibodies using clinical-grade samples of mobilized peripheral blood from 10 individual healthy donors under identical conditions. The CD133 negative fraction from the first selection was used for CD34… CONTINUE READING

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