Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples.

@article{Akens1997ComparativeAO,
  title={Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples.},
  author={Margarete K Akens and Edgar Holznagel and Marco Franchini and Verena Bracher},
  journal={Veterinary immunology and immunopathology},
  year={1997},
  volume={58 3-4},
  pages={
          231-7
        }
}
In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were used. There was no significant difference in the results obtained by these two methods.