Comparative analysis of cleavable azobenzene-based affinity tags for bioorthogonal chemical proteomics.

@article{Yang2010ComparativeAO,
  title={Comparative analysis of cleavable azobenzene-based affinity tags for bioorthogonal chemical proteomics.},
  author={Yu-ying Yang and Markus Grammel and Anuradha S Raghavan and Guillaume Charron and Howard C Hang},
  journal={Chemistry & biology},
  year={2010},
  volume={17 11},
  pages={1212-22}
}
The advances in bioorthogonal ligation methods have provided new opportunities for proteomic analysis of newly synthesized proteins, posttranslational modifications, and specific enzyme families using azide/alkyne-functionalized chemical reporters and activity-based probes. Efficient enrichment and elution of azide/alkyne-labeled proteins with selectively cleavable affinity tags are essential for protein identification and quantification applications. Here, we report the synthesis and… CONTINUE READING
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