Comparative analysis of antigen-targeting sequences used in DNA vaccines.

  title={Comparative analysis of antigen-targeting sequences used in DNA vaccines.},
  author={Joana A Carvalho and Adriano Rodrigues Azzoni and Duarte M. F. Prazeres and Gabriel Ant{\'o}nio Amaro Monteiro},
  journal={Molecular biotechnology},
  volume={44 3},
Plasmid vectors can be optimized by including specific signals that promote antigen targeting to the major antigen presentation and processing pathways, increasing the immunogenicity and potency of DNA vaccines. A pVAX1-based backbone was used to encode the Green Fluorescence Protein (GFP) reporter gene fused either to ISG (Invariant Surface Glycoprotein) or to TSA (trans-sialidase) Trypanosoma brucei genes. The plasmids were further engineered to carry antigen-targeting sequences, which… CONTINUE READING