Comparative analysis of Embden-Meyerhof and Entner-Doudoroff glycolytic pathways in hyperthermophilic archaea and the bacterium Thermotoga

  title={Comparative analysis of Embden-Meyerhof and Entner-Doudoroff glycolytic pathways in hyperthermophilic archaea and the bacterium Thermotoga},
  author={Martina Selig and Karina B. Xavier and Helena Santos and Peter Sch{\"o}nheit},
  journal={Archives of Microbiology},
The Embden-Meyerhof (EM) or Entner-Doudoroff (ED) pathways of sugar degradation were analyzed in representative species of the hyperthermophilic archaeal genera Thermococcus, Desulfurococcus, Thermoproteus, and Sulfolobus, and in the hyperthermophilic (eu)bacterial genus Thermotoga. The analyses included (1) determination of 13C-labeling patterns by 1H- and 13C-NMR spectroscopy of fermentation products derived from pyruvate after fermentation of specifically 13C-labeled glucose by cell… 
Molecular and biochemical characterization of bifunctional pyruvate decarboxylases and pyruvate ferredoxin oxidoreductases from Thermotoga maritima and Thermotoga hypogea.
The close relatedness of the PORs that was shown by sequence analysis could be an indication of the presence of such bifunctionality in other hyperthermophilic bacteria.
The thermophilic biomass-degrading bacterium Caldicellulosiruptor bescii utilizes two enzymes to oxidize glyceraldehyde 3-phosphate during glycolysis
Using metabolomics and kinetic analyses, it is shown that GOR functions in parallel with the conventional GAP dehydrogenase, providing an alternative ferredoxin-dependent glycolytic pathway and likely facilitates the recycling of reduced redox carriers (NADH and ferredox) in response to environmental H2 concentrations.
Hydrogen production by the hyperthermophilic bacterium Thermotoga maritima part I: effects of sulfured nutriments, with thiosulfate as model, on hydrogen production and growth
The stimulant effects of thiosulfate at very low concentrations on T. maritima growth have forced us to reconsider its role in this species and more probably also in all thiosolfato-reducer hyperthermophiles and to improve the hydrogen production in bio-processes using Thermotoga species, it should be recommended to incorporate thios sulfurate in the culture medium.
An essential role for tungsten in the ecology and evolution of a previously uncultivated lineage of anaerobic, thermophilic Archaea
The genome-guided cultivation of a member of the elusive archaeal lineage Caldarchaeales (syn. Aigarchaeota), Wolframiiraptor gerlachensis, is described and a crucial role for tungsten-dependent metabolism in the origin and evolution of this lineage is implied.
Carbohydrate Metabolism in Archaea: Current Insights into Unusual Enzymes and Pathways and Their Regulation
The aim of this review is to present the current understanding of central carbohydrate metabolic pathways and their regulation in Archaea as well as an overview focusing on hexose metabolic pathways identified in archaeal model organisms.
Profiling of glucose-induced transcription in Sulfolobus acidocaldarius DSM 639
Sulfolobus species can grow on a variety of organic compounds as carbon and energy sources. These species degrade glucose to pyruvate by the modified branched Entner–Doudoroff pathway. We attempted
Uncoupling Fermentative Synthesis of Molecular Hydrogen from Biomass Formation in Thermotoga maritima
Cell lines of the extremophile Thermotoga maritima are described that exceed the physiologic limits for H2 formation arising from genetic changes in fermentative metabolism, indicating that a reduced rate of maltose uptake was accompanied by an increase in H2 production, changing fermentation efficiency and shifting energy management.
Members of the Order Thermotogales: From Microbiology to Hydrogen Production
The ecology, phylogeny and metabolism linked to hydrogen production of these bacteria, are reviewed and members of the deep-branching order Thermotogales are recognized as high hydrogen producers having possible applications in biotechnology.


Evidence for the operation of a novel Embden-Meyerhof pathway that involves ADP-dependent kinases during sugar fermentation by Pyrococcus furiosus.
The main pathway for the fermentation of maltose or cellobiose by the hyperthermophile Pyrococcus furiosus was investigated by in vivo NMR and by enzyme measurements, and the first descriptions of ADP-dependent (AMP-forming) kinases to date are described.
Gluconeogenesis from pyruvate in the hyperthermophilic archaeon Pyrococcus furiosus: involvement of reactions of the Embden-Meyerhof pathway
Kinetic properties of the enzymes involved in gluconeogenesis indicate that they operate in the direction of sugar synthesis, whereas sugar degradation to pyruvate proceeds via a modified “non-phosphorylated” Entner-Doudoroff pathway.
Maltose fermentation to acetate, CO2 and H2 in the anaerobic hyperthermophilic archaeon Pyrococcus furiosus: evidence for the operation of a novel sugar fermentation pathway
This novel pathway of maltose fermentation to acetate, CO2 and H2 in the anaerobic archaeon P. furiosus may represent a phylogenetically ancient pathway of sugar fermentation.
Glucose catabolism of the hyperthermophilic archaeum Thermoproteus tenax
Pulse labelling experiments and enzyme studies show that Thermoproteus tenax is able to degrade glucose via the Embden-Meyerhof-Parnas (EMP) pathway, indicating that under chemoorganotrophic conditions at least 2 glycolytic pathways are operative in T. tenax.
Acetyl-CoA synthetase (ADP forming) in archaea, a novel enzyme involved in acetate formation and ATP synthesis
Data indicate that acetyl-CoA synthetase (ADP forming) represents a typical archaeal property rather than an enzyme specific for hyperthermophiles, whereas in all acetate forming (eu)bacteria these reactions are catalyzed by two enzymes, phosphate acetyltransferase and acetate kinase.
Glucose metabolism in the extreme thermoacidophilic archaebacterium Sulfolobus solfataricus.
The oxidative breakdown of glucose to pyruvate occurring in S. solfataricus differs from the Entner-Doudoroff pattern in that there is an absence of any phosphorylation step.
Glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus woesei: characterization of the enzyme, cloning and sequencing of the gene, and expression in Escherichia coli
The glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus woesei was purified to homogeneity and exhibited a strikingly high proportion of aromatic amino acid residues and a low proportion of sulfur-containing residues.
Glyceraldehyde-3-phosphate Ferredoxin Oxidoreductase, a Novel Tungsten-containing Enzyme with a Potential Glycolytic Role in the Hyperthermophilic Archaeon Pyrococcus furiosus(*)
It is proposed that GAPOR has a glycolytic role and functions in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase.
Formation of l-alanine as a reduced end product in carbohydrate fermentation by the hyperthermophilic archaeon Pyrococcus furiosus
Alanine formation was shown to occur via an alanine aminotransferase, which exhibited a specific activity in cell-free extract of up to 6.0 U/mg (90°C; direction of pyruvate formation), enabling P. furiosus to adjust its fermentation, depending on the redox potential of the terminal electron acceptor.
Chapter 1 Central metabolism of the archaea