The aim of this work liras to determine the different anthraquinoneS in Aloe Second!flora, an indigenous species of Aloe growing around Pharmacy Department (Kenyatta ftSationel Hospital). This was composed with the anthraquininei preset*« in cape Aloe species which is mainly produced from Cape Province(South Africa) First, chemical method was used to determine the quantities cf the enthraquinones by vusually comparing the intesities of colour produced in the two species. ■ Chemical methods ehoysd that the Cape species had more anthraquinone than the Secundiflora. The Anthraquinor.es were then asperated using thin layer chromatography method, both species being run on the same plate using same developing solvent that had previously been selected by trying different combination of solvents on glass slides. The Rf values were coroparrablc; the conditions having been reasonably the same in the Developing chamber. The spota on TaL*C* wore recovered using chloroform, which anthraquinones ard' soluble in. The chloroform portion was filtered and then run on Infra-red machine to try and determine any structural differences that resulted In the separation into spots of different heights.