Comparative Sequence-Function Analysis of the Major Facilitator Superfamily: The "Mix-and-Match" Method.

@article{Madej2015ComparativeSA,
  title={Comparative Sequence-Function Analysis of the Major Facilitator Superfamily: The "Mix-and-Match" Method.},
  author={M Gregor Madej},
  journal={Methods in enzymology},
  year={2015},
  volume={557},
  pages={521-49}
}
The major facilitator superfamily (MFS) is a diverse group of secondary transporters with members found in all kingdoms of life. The paradigm for MFS is the lactose permease (LacY) of Escherichia coli, which has been the test bed for the development of many methods applied for the analysis of transport proteins. X-ray structures of an inward-facing… CONTINUE READING