Comparative Lipidomics and Proteomics of Lipid Droplets in the Mesocarp and Seed Tissues of Chinese Tallow (Triadica sebifera)

@article{Zhi2017ComparativeLA,
  title={Comparative Lipidomics and Proteomics of Lipid Droplets in the Mesocarp and Seed Tissues of Chinese Tallow (Triadica sebifera)},
  author={Yao Zhi and Matthew C. Taylor and Peter M. Campbell and Andrew C. Warden and Pushkar Shrestha and Anna El Tahchy and Vivien Rolland and Thomas Vanhercke and James Robertson Petrie and Rosemary G. White and Wenli Chen and Surinder Pal Singh and Qing Liu},
  journal={Frontiers in Plant Science},
  year={2017},
  volume={8}
}
Lipid droplets (LDs) are composed of a monolayer of phospholipids (PLs), surrounding a core of non-polar lipids that consist mostly of triacylglycerols (TAGs) and to a lesser extent diacylglycerols. In this study, lipidome analysis illustrated striking differences in non-polar lipids and PL species between LDs derived from Triadica sebifera seed kernels and mesocarp. In mesocarp LDs, the most abundant species of TAG contained one C18:1 and two C16:0 and fatty acids, while TAGs containing three… 
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References

SHOWING 1-10 OF 107 REFERENCES
Oil biosynthesis in a basal angiosperm: transcriptome analysis of Persea Americana mesocarp
TLDR
This work represents a comprehensive transcriptome resource for a basal angiosperm species and provides insight into their lipid metabolism in mesocarp tissues and has implications for metabolic engineering to enhance storage oil content in nonseed tissues of diverse species.
Biogenesis and functions of lipid droplets in plants
TLDR
Several other proteins known to be important inLD biogenesis in yeasts and mammals are conserved in plants, suggesting that at least some aspects of LD biogenesis and/or function are evolutionarily conserved.
Subcellular Lipid Droplets in Vanilla Leaf Epidermis and Avocado Mesocarp Are Coated with Oleosins of Distinct Phylogenic Lineages1[OPEN]
TLDR
Two new types of oleosin-LDs exemplify the evolutionary plasticity of oLEosins- LDs in generating novel functions in diverse cell types and species.
Oleosin of Subcellular Lipid Droplets Evolved in Green Algae1[W][OA]
TLDR
Overall, oleosin genes having weak and cell/development-specific expression were present in green algae and a hypothesis for the evolution of oleOSins from algae to plants is presented.
Deep Sequencing of the Fruit Transcriptome and Lipid Accumulation in a Non-Seed Tissue of Chinese Tallow, a Potential Biofuel Crop.
TLDR
This study shows that tallow is in fact a non-seed cellular tissue capable of triglyceride synthesis, and describes the annotated transcriptome assembly generated from the fruit coat, tallow and seed tissues of Chinese tallow.
Lipid droplet-associated proteins (LDAPs) are involved in the compartmentalization of lipophilic compounds in plant cells
TLDR
Evidence is provided for a role of LDAPs in lipid accumulation in oil-rich fruit tissues, and it is proposed that the larger LDAP/SRPP protein family plays important roles in the compartmentalization of lipophilic compounds, including triacylglycerols and polyisoprenoids, into lipid droplets within plant cells.
Protein and lipid composition analysis of oil bodies from two Brassica napus cultivars
TLDR
Two new proteins were identified in this study, one of which is similar to a triacylglycerol‐associated factor from narrow‐leafed lupin while the other, a protein annotated as a myrosinase associated protein, shows high similarity to the lipase/hydrolase family of enzymes with GDSL‐motifs.
Purification and characterization of oil-bodies (oleosomes) and oil-body boundary proteins (oleosins) from the developing cotyledons of sunflower (Helianthus annuus L.)
TLDR
The oil-body surface appears to be an unusual and novel structure, covered largely by an oleosin protein coat or pellicle rather than a conventional fluid membrane, half-unit or otherwise.
Identification of a New Class of Lipid Droplet-Associated Proteins in Plants1[C][W][OPEN]
TLDR
It is proposed that small rubber particle protein-like proteins are involved in the general process of binding and perhaps the stabilization of lipid-rich particles in the cytosol of plant cells and that the avocado and Arabidopsis protein members reveal a new aspect of the cellular machinery that is involved inThe packaging of triacylglycerols in plant tissues.
...
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