Comparative Analyses of the Secretome from Dedifferentiated and Redifferentiated Adult Articular Chondrocytes

@inproceedings{Polacek2011ComparativeAO,
  title={Comparative Analyses of the Secretome from Dedifferentiated and Redifferentiated Adult Articular Chondrocytes},
  author={Martin Polacek and Jack-Ansgar Bruun and Oddmund Johansen and I{\~n}igo Martinez},
  booktitle={Cartilage},
  year={2011}
}
OBJECTIVE The main goal of this study was to compare the secretion products derived from human articular chondrocytes established in either long-term monolayer cultures or in scaffold-free 3-dimensional (3-D) cultures. METHODS Stable isotope labeling of amino acids in cell culture (SILAC) was applied to investigate quantitatively the differences between proteins secreted from dedifferentiated and redifferentiated chondrocytes. Proteins in cell supernatants were resolved by 1-D gel… CONTINUE READING