Compact detector for proteins based on two-photon excitation of native fluorescence.

@article{Paul2005CompactDF,
  title={Compact detector for proteins based on two-photon excitation of native fluorescence.},
  author={Uchenna Prince Paul and Li Li and Milton L. Lee and Paul B. Farnsworth},
  journal={Analytical chemistry},
  year={2005},
  volume={77 11},
  pages={
          3690-3
        }
}
A compact, inexpensive detector for proteins has been constructed based on two-photon excitation of fluorescence from phenylalanine, tyrosine, and tryptophan. The fluorescence was excited by a solid-state microchip laser operating at 532 nm. Detection limits for phenylalanine, tyrosine, and tryptophan were 62 microM, 2.0 microM, and 470 nM, respectively, in a volume of 3 fL. The detection limit for a test protein, bovine serum albumin, was 130 nM.