Community structure of the microbiota in the floodwater of a Japanese paddy field estimated by restriction fragment length polymorphism and denaturing gradient gel electrophoresis pattern analyses

@article{Kimura2002CommunitySO,
  title={Community structure of the microbiota in the floodwater of a Japanese paddy field estimated by restriction fragment length polymorphism and denaturing gradient gel electrophoresis pattern analyses},
  author={Makoto Kimura and Taketoshi Shibagaki and Yasunori Nakajima and Kazuo Matsuya and Makoto Ikenaga},
  journal={Biology and Fertility of Soils},
  year={2002},
  volume={36},
  pages={306-312}
}
The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR-denaturing gradient gel electrophoresis (DGGE) patterns of 16S rDNA were studied to elucidate the effects of the type of fertilization and the growth stage of rice plants on the community structure of the microbiota in the floodwater of a Japanese paddy field under a long-term fertilizer trial. From the mid tillering stage, a higher pH and temperature were observed in the plot without fertilization (NoF… CONTINUE READING

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